Test Method 168-2012
Agents: Active Ingredient Content of Polyaminopolycarboxylic
Acids and Their Salts; Copper PAN Method
in 1987 by AATCC Committee RA90
Purpose and Scope
This test method is an alternate to AATCC Method 149, Chelating Agents: Chelation
Value of Aminopolycarboxylic Acids and Their Salts; Calcium Oxalate Method.
1.2 The active content of ethylenefiaminetetraacetic acid (EDTA), N-hydroxyethylenediaminetriacetic
acid (HEDTA) and diethylenetriaminepentaacetic acid (DTPA) and their salts is
usually expressed by the calcium chelation value (CaCV). Titration with
calcium salt using either oxalate or a dye indicator, as done in AATCC Method
149, yields a value which includes partially substituted EDTA, HEDTA, DTPA, nitrilotriacetic
acid (NTA), iminodiacteic acid (IDA), glycolate and other weak chelating agents
found in some commercial products. This alternative procedure excludes the
effect of partially substituted products, providing a more stringent value.
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